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Caspase-3 activity was quantified in nanomoles of 7-Amino-4-methylcoumarin (the fluorescent product) liberated from acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin per minute per milliliter of cell lysate, calculated using the formula: Where v = volume of the sample in milliliters, d = dilution factor, and t = reaction time in min

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