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After several rounds of heterogeneous refinement, 80,325 particles were used for subsequent NU-refinement, resulting in a 3.70 resolution of the ATD 3D reconstruction

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Bulk RNA Seq RNA quality was determined by RNA integrity number with Agilent Technologies 2100 Bioanalyzer (Cat #5067-1511), to confirm that all samples had an RNA integrity number exceeding 9 and were deemed suitable for further analysis