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We cross-referenced coimmunoprecipitation and proximity labeling data sets, which accounted for three different NHR-49 strains including the overexpressed all-tissue NHR-49::GFP under fed and fasted conditions 42 and the endogenous N- and C-terminal TurboID tagged NHR-49 48
Cys 2 ), cystine-free (Cys 2 -free), or cystine-free/GSH-supplemented (750 M) medium for the indicated timepoints were determined
these determine the final concentration and must not be estimated
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