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Following that, youll want to take the following steps under their supervision [5]

The reagent mixture was incubated for 3 min at 37C and the reaction was initiated by addition of (final concentration) 60 M peroxides, at a total volume of 0.5 mL

Some source-lab COAs may include limited redactions to protect upstream laboratory confidentiality and prevent unauthorized or unsolicited contact with source-lab partners

(C and D) Structural models of the catalytic site of Cu(II)-free tyrosinase, in which the side chain of the His54 residue takes conformations orienting toward the CuA-binding site and the surface of the caddie protein, respectively