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This reaction has two consequences studied in the literature: it consumes S-adenosyl methionine, the cells principal methyl donor, and it removes nicotinamide from the salvage pathway that regenerates NAD+

NNMT inhibition preserved nicotinamide substrate pool Reduced nicotinamide methylation conserved S-adenosyl methionine Elevated intracellular NAD+ increased sirtuin pathway activity Adipocyte metabolic shift altered lipid handling in cell models Core Areas of Scientific Investigation NNMT Enzyme Inhibition: NNMT activity is elevated in adipose tissue in obesity models